We next verified their regulatory roles by cloning these four regions into the enhancer region of a luciferase reporter, and observed that the E2 region inhibited ATP5MD promoter activities, whereas the E3 region remarkably enhanced ATP5MD promoter activity in human SK-N-SH neuroblastoma cell line (Fig. 3a and Supplementary Fig. 10). This evidence concerns the gene ATP5MK and neuroblastoma.