The aim of this study was to identify potentially new damaging variants in the above-mentioned four gene candidates by analyzing large cohort of the MODY patients, which lacks detrimental mutations in any of the 12 main MODY genes (HNF4A, GCK, HNF1A, PDX1, HNF1B, NEUROD1, CEL, INS, ABCC8, KCNJ11, APPL1, and BLK). This evidence concerns the gene INS and MODY.