Both GSCs and GBs can be identified by anti-Vasa antibody staining, and meantime by carrying spherical fusomes (spectrosomes) (Fig. 1a), and GBs undergo four times of successive cell division and generates a 16-cell germline cyst, interconnected by a branched fusome that can be visualized by anti-Hts antibody (Fig. 1a,b). The gene discussed is DDX4; the disease is cyst.