MiR-328-5p mimics decreased the levels of cell cycle proteins, cyclin-D1 and CDK4, and increased the levels of pro-apoptotic proteins (cleaved Caspase3 and Bax) in ESCC cells, whereas, miR-328-5p inhibitors reversed these effects (Figure 4C–4E). This evidence concerns the gene CCND1 and esophageal squamous cell carcinoma.