Differences in expression of MYCN and MYC in our NB cell line panel were confirmed by western blot in line with literature (online supplemental figure 1C).28 Depletion of MYCN by RNAi moderately reduced the levels of EHMT2 and EZH2 but also EHMT1 and EZH1 (figure 1E–G). This evidence concerns the gene EHMT1 and neuroblastoma.