Moreover, through signal pathway enrichment analysis and verification based on western blot assay, we confirmed that eIF6 could activate mTOR-related cancer signaling pathways and thereby regulate downstream genes, such as MYC, CDK4, CDK6, CCND1, CASP3 and CTNNBL1, to affect cell proliferation, the cell cycle, apoptosis and invasion in HCC. The gene discussed is CASP3; the disease is hepatocellular carcinoma.