CDK4 and hepatocellular carcinoma: Moreover, through signal pathway enrichment analysis and verification based on western blot assay, we confirmed that eIF6 could activate mTOR-related cancer signaling pathways and thereby regulate downstream genes, such as MYC, CDK4, CDK6, CCND1, CASP3 and CTNNBL1, to affect cell proliferation, the cell cycle, apoptosis and invasion in HCC.