The expression of TPD52, C1orf210, LOC100129620, and LOC102724009 was significantly higher in extracted lung metastasis tissue than in in situ osteosarcoma tissue (Figure 1B and Supplementary Figure 1A), while the expression of APBB1IP, HORMAD1, ST5, and LOC101930400 was significantly lower in lung metastasis tissue than in orthotopic osteosarcoma tissue (Supplementary Figure 1B). Here, HORMAD1 is linked to osteosarcoma.