To validate the disproportionate expression levels between NKX2‐1/TTF‐1 exon 1 and exon 4/5 observed in FFPE tumour tissues, expression of both NKX2‐1/TTF‐1 exon 1 and exon 4/5 was evaluated in a panel of 10 lung cancer cell lines, consisting of six EGFR‐mutated and four KRAS‐mutated cell lines. This evidence concerns the gene RHOH and lung carcinoma.