circAGFG1 silencing downregulated the expression of glycolytic crucial enzymes (GLUT1, PGK1, and PKM2) and reduced the uptake of glucose and the production of lactate and ATP, and the silencing of miR-28-5p or the overexpression of HIF-1α largely rescued the glycolysis of NSCLC cells, suggesting that circAGFG1 accelerated the glycolysis of NSCLC cells partly through miR-28-5p/HIF-1α axis. This evidence concerns the gene PKM and non-small cell lung carcinoma.