We observed that the CLL clone maintained CD19 and CD5 expression during the whole culture period, with mean values of 93%, 89%, 89%, 87%, 95% for day 0-7-14-21-28, respectively (Figures 7A, B and Supplementary Figures 9A, B) (n = 15). This evidence concerns the gene CD5 and B-cell chronic lymphocytic leukemia.