Moreover, we did not observe malignant transformations in the livers of AAV- or LNP-treated mice after 18 and 25 weeks, and prolonged ABE expression over 1 year in mice sensitized to tumor development by liver-specific Trp53 deletion42,43 did not enhance hepatocellular carcinoma (HCC) formation (Fig. 3e,f and Supplementary Fig. 8a–c). This evidence concerns the gene TP53 and hepatocellular carcinoma.