To perform these analyses, the phenotypes of fkrp−/− and dag1−/− mutants were  compared for muscle structure and integrity defects at 3 and 5 days post fertilisation (dpf), a period during which progressive muscular dystrophy and muscle basement membrane (MBM) attachment defects were evident in our previously generated zebrafish models of muscular dystrophy11–13. Here, DAG1 is linked to progressive muscular dystrophy.