The top hits with increased ubiquitination in DCM included myosin regulatory light chain-2, desmin, myozenin-2, α-actinin-2, and tropomyosin alpha-1, which had significantly higher ubiquitination when analyzed by spectral count compared to nonfailing (Fig. 1h–n). This evidence concerns the gene MYOZ2 and familial dilated cardiomyopathy.