Although the RAF-MEK-ERK and PI3K-AKT-mTOR cascades have been reported to be accelerated in neurofibromatosis 13,14,15, we could not detect significant differences in the phosphorylation levels of RAF, MEK, ERK, and AKT between HEFs and NFFs (Supplementary Fig. S2). Here, MAP2K7 is linked to neurofibromatosis.