Although the RAF-MEK-ERK and PI3K-AKT-mTOR cascades have been reported to be accelerated in neurofibromatosis 13,14,15, we could not detect significant differences in the phosphorylation levels of RAF, MEK, ERK, and AKT between HEFs and NFFs (Supplementary Fig. S2). The gene discussed is AKT1; the disease is neurofibromatosis.