As a result, methyltransferase-like 14 (METTL14) exhibited significantly decreased mRNA level in osteosarcoma samples with metastasis compared with those without metastasis in the training set as shown in Figure 1B. Another 2 regulators including fat mass and obesity-associated protein (FTO), and zinc finger CCCH domain-containing protein 13 (ZC3H13) could achieve marginal significance in the training set. The gene discussed is FTO; the disease is osteosarcoma.