Transactivation of iNOS expression and the iNOS-S100A8/A9 signaling axis in Hltf-deleted TME drove the comparison of S-nitroso-proteome of primary tumor with metastatic tumors in Hltf-deleted TMEs to reveal remarkable spatiotemporal continuity in S-nitrosylation signaling. This evidence concerns the gene HLTF and metastatic neoplasm.