CD40LG and Zika virus infectious disease: They are: a) presenting negative IgM serology five days before the procedure; b) wait up to 90 days after the appearance of signs and symptoms associated with Zika virus infection when the man was infected or report risky sexual exposure or travel to endemic regions; c) wait up to 60 days after signs/symptoms associated with Zika virus infection when the woman was infected or report risky sexual exposure or travel to regions endemic for this infection25,63,75,.76