Similar results were obtained from western blot analysis of mice melanoma tumor lysates treated with different doses of PCFE which showed significant downregulation of CD133 (p < 0.0001; F ratio = 70.74; R2 = 0.963) and vimentin (p < 0.0001; F ratio = 152; R2 = 0.982) and upregulation of E-cadherin expression (p < 0.0001; F ratio = 158.1; R2 = 0.983) (Figures 6(b) and 6(c)). This evidence concerns the gene VIM and neoplasm.