As shown in Figure 3, compared with the normal control group, NAFLD rats showed elevated serum levels of ALT (Figure 3(a)), AST (Figure 3(b)), CR (Figure 3(c)), and BUN (Figure 3(c)) (P < 0.05), respectively, which suggested that HFD caused hepatic and renal injury in NAFLD rats. This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.