To sum up, the results of this study showed the effect of RAS-RH on mitigating X-irradiation-induced myocardial fibrosis in vitro via attenuating the expression of TGF-β1, Col1, and α-SMA, and blocking the sustainable injury of cardiac fibrosis exposed to X-ray radiation. The gene discussed is ACTA1; the disease is Myocardial fibrosis.