To further clarify the correlation between DDIT4 expression level and autophagy in pancreatic cancer tissues, we performed immunofluorescence on pancreatic cancer tissues which confirmed that the expression level of LC3 and DDIT4 was significantly high in pancreatic cancer at the same time compared to the control (the low expression level of DDIT4). Here, MAP1LC3A is linked to familial pancreatic carcinoma.