The MFIs of FITC-labeled anti-HMGB1 antibodies in the primary tumor tissues of SPNpro-, SPN-1-, or SPN-2-injected mice were greatly increased by 30.6-, 25.0-, and 26.7-fold relative to that for the saline-injected mice, respectively (Fig. 5d, Supplementary Fig. 21a). This evidence concerns the gene HMGB1 and neoplasm.