NIPBL and Cornelia de Lange syndrome: Taken together with previous data demonstrating the impact of Nipbl heterozygosity on cohesin binding and 3D chromatin contacts31–34, our analysis supports the idea that deregulation both in CdLS in vivo and in in vitro models for acute cohesin cleavage identifies a core set of genes that are enriched for binding of CTCF and cohesin, and that show enhancer-dependent tissue-specific expression.