Protein levels of typical indicators were also detected by western blotting, which showed that miR-224-5p introduction significantly reduced protein levels of Ki67, PCNA, BCL-2, and p-ERK/ERK, but dramatically increased cleaved caspase-3 levels in the AML cells overexpressing CD27-AS1 (Fig. 6f, g). This evidence concerns the gene PCNA and acute myeloid leukemia.