Although WT parasite infection induces secretion of IL-1β compared with the uninfected BMDM (Fig. 5A), infection with Δmag1 parasites further doubles the secretion of IL-1β compared with WT parasites (P < 0.001; analysis of variance [ANOVA] with Tukey’s honestly significant difference [HSD] test). This evidence concerns the gene IL1B and parasitic infectious disease.