Overall, our findings reveal the previously uncharacterized anti-tumor effect of ASMTL-AS1 in TNBC, in which ASMTL-AS1 acts as a ceRNA sponging miR-1228-3p and elevating SOX17, resulting in the inactivation of Wnt/β-catenin signaling, thereby inhibiting TNBC tumorigenesis and progression. The gene discussed is SOX17; the disease is neoplasm.