Our data show a significant decrease in l‐glutamine and l‐leucine uptake levels following the knockdown of SLC1A5/ASCT2, SLC7A5/LAT1, and SLC38A2/SNAT2, indicating that these transporters fulfills the AA requirements of CRC cells in a coordinated fashion. The gene discussed is SLC7A5; the disease is colorectal carcinoma.