Collectively, our data show that the selected AATs (SLC1A5/ASCT2, SLC7A5/LAT1, and SLC38A2/SNAT2) play important roles in the activation of mTOR and CRC cell proliferation, by facilitating uptake of amino acids such as l‐glutamine and l‐leucine. This evidence concerns the gene SLC7A5 and colorectal carcinoma.