MIEN1 and neoplasm: Four tumours that had a strongly amplified Neu locus by qPCR, all of which were AC(NST), showed amplification by exome data of a segment in chromosome 11 that included Erbb2 but also nearby genes Mien1 and Grb7. Two tumours also had amplified loci on chromosome 19, which included Pten and Atad1. In four tumours [two ASQCs, one an AC(NST) and a mammary sarcoma], all of which had low levels of amplification by qPCR, no CNVs passing the statistical threshold could be detected (Fig. 3B; Tables S8 and S9).