Especially, experimental validation these potential splicing factors (e.g. SPEN, SF3B5, RNPC3, LUC7L3, SRSF11, and PRPF38B) regulate alternative splicing events, and it is also important to use experimental validation of wet analysis (RT-PCR) and protein level, and consider the fact that different portions of the tumour have different expression and splicing profiles as well as the importance of the micro-environment. Here, SPEN is linked to neoplasm.