INS and glucose measurement: While persistently hyperphagic db/db mice that overexpressed Yap in striated muscles continued to exhibit fasting hyperglycaemia and impaired whole-body glucose tolerance (Supplementary Fig. 11A, B), fasting plasma concentrations of insulin (Fig. 5e) and c-peptide levels (Supplementary Fig. 11C) were lower indicative of a reduction in insulin secretion.