Evaluation of the expanded cohort confirmed the status of IL13Rα2 overexpression, with the combined new dataset and previous dataset (n = 28 DIPG tumor samples, n = 18 normal tissue samples) demonstrating 21-fold overexpression in matched tumor vs. normal brain tissue and 12-fold overexpression in all tumor vs. normal samples (p < 0.0001) (Fig. 1a, b), remaining significantly higher (p = 0.01) than the tumor-normal expression ratio of IL13Rα1 (Fig. 1b, c). Here, IL13RA1 is linked to neoplasm.