To further demonstrate the effect of the CCL5/CCR5 pathway on the stemness of PCa cells in the co-culture system via colony formation and sphere formation, we treated PC-3 cells with anothernon-peptidesyntheticCCR5antagonist, TAK-779, {N,N-dimethyl-N-[4-[[[2-(4-methylphenyl)-6,7-dihydro-5H-benzo-cyclohepten-8-yl]car bon-yl]amino]benzyl]-tetra-hydro-2H-pyran4-aminium chloride, which inhibits hCCR5 by inducing a direct blockade of ligand binding and not by the internalization of hCCR5. This evidence concerns the gene CCR5 and posterior cortical atrophy.