ASCL1 and adenocarcinoma: Comparative RNA-seq studies and immunohistochemical staining revealed a strong KCTD16 expression in H69 cells (ASCL1-positive, classical type SCLC) and in ASCL1-transfected A549 adenocarcinoma cell lines, while in H69AR (ASCL1-negative, variant type SCLC) and A549 (control) cell lines the level of KCTD16 protein does not result upregulated [104].