Further RNA-seq analysis indicated that a series of metastasis and invasion molecules were upregulated in PRICKLE1-high AML patients, such as CCL22, CCL23, CCL24, MMP2, MMP7, MMP14, MMP19 and S100A1; while invasion suppressors, such as CDH1 [27], were downregulated in PRICKLE1high AML patients (Fig. 7c and Additional file 1: Table S3). The gene discussed is MMP14; the disease is acute myeloid leukemia.