To further verify RNA-Seq’s results, we used RT-qPCR to identify some gene’s expression that were significantly changed in RNA-seq and related to tumor progression, RT-qPCR showed that after interfering with KDM6B, the expression of CLDN16,MMP28,CXCL2,BCL6, JUNB, STAT3,STAT5A and C/EBPβ mRNA was lower than that of the control group. This evidence concerns the gene KDM6B and neoplasm.