Coincidence with CCK8 assay, the Interference of KDM6B also significantly decreased the clone formation ability of KYSE150 and TE10 cells, and the overexpression of KDM6B in Eca9706 and TE11 promoted the clone formation ability of ESCC cells (Fig. 2c, Sup Fig. 1C). The gene discussed is KDM6B; the disease is esophageal squamous cell carcinoma.