Peripheral unilateral injection of BoNT/A could inhibit bilateral trigeminal neuropathic pain and anxiety-like behaviors, which is associated with the inhibition of microglia activation, the upregulation of expression of TLR2, its downstream of MyD88 and proinflammatory factors in the ipsilateral TNC and inhibit the activation of neurons in the contralateral TNC following dIoN-CCI surgery in mice. This evidence concerns the gene TLR2 and Anxiety.