We also measured VEGF secreted by pericytes and astrocytes post-OGD reperfusion as elevations in VEGF are correlated with increased endothelial barrier permeability post-ischemia.45–47 Li and co-authors found that astrocyte-derived VEGF mediated endothelial barrier disruption, which was associated with decreases in occludin and claudin-5.47 Interestingly, whilst CBG and CBDV did not affect pericyte-derived VEGF, CBDV decreased VEGF secretion in astrocytes in a concentration-dependent manner and CBG exhibited a trend for decreasing VEGF at 100 nM and 3 μM. The gene discussed is CLDN5; the disease is ischemia.