Our study further expands the genetic landscape of inherited variants of HL-associated genes in the Pakistani population and revealed three novel variants in three known nonsyndromic deafness genes, MYO15A, PTPRQ, and SERPINB6. Although variants in MYO15A are a commonly known cause of HL worldwide (including the Pakistani population) [16], variants of PTPRQ and SERPINB6 are relatively rare. Here, PTPRQ is linked to deafness.