However, a dietary protein preload augments carbohydrate-induced insulin secretion in patients with T2DM (8), whereas short-term dietary BCAA restriction reduces plasma BCAA concentrations and attenuates postprandial insulin and C-peptide concentrations after ingestion of a mixed meal in patients with obesity and T2DM (9). This evidence concerns the gene INS and obesity due to melanocortin 4 receptor deficiency.