Consistent with previous studies (23, 24), the pathway analysis of somatic variants for tumor DNA showed that alterations in either FGFR3 or the receptor tyrosine kinase/phosphatidylinositol 3-kinase pathway were identified more frequently in LG-NIMBC in comparison to HG-NIMBC, whereas variations in either TP53 or cell cycle regulation genes were more common in HG disease than in LG disease. This evidence concerns the gene NTRK1 and neoplasm.