Western-blot experiment showed an increased cleaved caspase-3 expression and decreased antiapoptotic protein Bcl-2 expression (Figures 4A, B), along with the increased autophagic protein Beclin-1 and autophagy marker protein LC3B expression (Figures 4C, D), when NGFR overexpression CRC cells treated with 5-FU. The gene discussed is BCL2; the disease is colorectal carcinoma.