As a traditional malignant tumor phenotype, the gene signature of the invasion group consisted of NHSL1, OCLN, EPB41L4B, PLAUR, PIK3CB, EPB41L5, ID4, TPM4, and MARVELD2. TPM4 showed the opposite effects in colon cancer and lung cancer. Here, EPB41L4B is linked to malignant colon neoplasm.