In conclusion, we demonstrated mutations in several factors related to HCC that had not been previously identified, such as TMEM141, A disintegrin and metalloproteinase with thrombospondin type 1 motif, 9 (ADAMTS9), and ADGRV1. Of these, ADGRV1 mutations were most critical, because frameshift deletion variants and frameshift insertion variants were found contained in ADGRV1. Here, ADGRV1 is linked to hepatocellular carcinoma.