Rasmussen et al. [9] analyzed intra-tumor PD-L1 expression heterogeneity in these cancers and reported a negative predictive value of single biopsies, with a tumor proportion score (using a 1% cut-off value) of 38.9%; however, this score increased to 79.9/56.8% when the evaluation used a 50% cut-off value with double biopsies, respectively. This evidence concerns the gene CD274 and cancer.