SREBF2 and colorectal carcinoma: To further explore the role of SREBP2-dependent cholesterol biosynthesis in colonization of metastatic CRC cells in the liver, we utilized another experimental CRC liver metastasis model by intrasplenic injection of HT29 cells transfected with non-target shRNA control (shNT) or HT29 SREBP2 knockdown cells using BALB/c nude mice (Figure 4D).