Similar to EEH experiments at 24 hpi, 20 μg/mL of ECB and 1 μM of PTK were added to the embryo medium of the zebrafish/melanoma xenograft, and tumor-induced angiogenesis was quantified by evaluating the number and length of ectopic vessels in the SIV of zebrafish/tumor xenografts at 24 and 48 hpi (Figure 2). The gene discussed is PTK2B; the disease is melanoma.