Although in our small study there was a statistically significant increase in CD4, CD14, CD68, CD8A, B7-H3, PDL-1, CD19, FOXP3, CD44, and STAT3 protein expression in methylated versus unmethylated GBM tumour core (see Table 2), we need to be cautions with these results due to the small sample size that requires further exploration and validation in larger cohorts using DSP. The gene discussed is CD4; the disease is glioblastoma.