In our study, VISTA mean normalised counts were on the higher end of the normalised values' range in both MGMT methylated and unmethylated IDH-wild-type GBM; however, there was no significant difference between groups: MGMT methylated tumour core 11.94 [0.70], MGMT unmethylated tumour core 11.42 [0.77]. This evidence concerns the gene MGMT and neoplasm.