CTSB and colitis: Further, to confirm that rTsSp was a particular T. spiralis protein that had the immune protective effects in mouse colitis model, we observed the body weight change of mice from another recombinant protein (a newborn larvae-specific cysteine protease inhibitor from Trichinella spiralis, with a predicted molecular weight of 46.9 kDa, expressed in pET-22b (+) plasmid and purified in our lab) pre-administration-DSS group or protein non-derived from helminth pre-administration-DSS group, and found that there was no immune protective effect stemming from those proteins (Supplementary Figure 3).