GAPDH and myocardial ischemia: Furthermore, based on the common DEPs which were regulated in ISO-induced myocardial ischemia rats and back-regulated in SMI pretreatment myocardial ischemia rats, we identified three serum biomarkers of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Fas apoptotic inhibitory molecule 3 (FAIM3), and uncharacterized protein (M0R5J4) verified by the PRM analysis.