Therefore, we used Itaconate to inhibit the enzymatic activity of ALDOA (Figure 5A) without changing the protein expression level of ALDOA (Figure 5B), and tested the proliferation and invasion abilities of ICC cells by methods such as CCK-8 and Transwell (Figure 5C-E, Supplementary Figure 4). This evidence concerns the gene ALDOA and intrahepatic cholangiocarcinoma.